EST-SSR Marker-Based Assay for Purity Identification of Melon “Green Angle”

In order to define the purity of melon (Cucumis melo L.) "Green Angle" variety F1 seed, PCR amplification, detection, and analysis were carried out with leaf DNA, using EST-SSR molecular marker technology. The results show that, among 57 SSR primers, PCR products with one pair of primer showed polymorphism from parents. For this marker, parents showed single allele whereas the hybrids showed both the parental alleles indicating the heterozygosity of the hybrids. 51 seeds were tested using the marker, and one seed was off-type. The same lot of seeds was also tested in the field and the purity was 98.4 %. It showed that the EST-SSR marker-based PCR assay is effective in determining the purity of F1-hybrid seeds of melon "Green Angle".