Fabrication of multi-well platform with electrical stimulation for efficient myogenic commitment of C2C12 cells
BIOMATERIALS AND BIOMECHANICS IN BIOENGINEERING(2015)
Abstract
To engineer tissue-like structures, cells are required to organize themselves into three-dimensional networks that mimic the native tissue micro-architecture. Here, we present agarose-based multi-well platform incorporated with electrical stimulation to build skeletal muscle-like tissues in a facile and highly reproducible fashion. Electrical stimulation of C2C12 cells encapsulated in collagen/matrigel hydrogels facilitated the formation 3D muscle tissues. Consequently, we confirmed the transcriptional upregulations of myogenic related genes in the electrical stimulation group compared to non-stimulated control group in our multi-well 3D culture platform. Given the robust fabrication, engineered muscle tissues in multi-well platform may find their use in high-throughput biological studies drug screenings.
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Key words
C2C12,electrical stimulation,agarose,based multi,well platform,collagen,matrigel,encapsulation
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