Separation Strategies for Proteomics

Abstract Originally published in: Analysing Gene Expression. Edited by Stefan Lorkowski, Paul Cullen. Copyright © 2003 Wiley‐VCH Verlag GmbH & Co. KGaA Weinheim. Print ISBN: 3‐527‐30488‐2 The sections in this article are Introduction Conventional slab gel electrophoresis Isoelectric focusing ( IEF ) Sodium dodecyl sulphate polyacrylamide gel electrophoresis ( SDS ‐ PAGE ) and Western blotting Two‐dimensional polyacrylamide gel electrophoresis (2 D ‐ PAGE ) Two‐dimensional difference gel electrophoresis (2 D ‐ DIGE ) High‐performance liquid chromatography ( HPLC ) Ion exchange chromatography ( IEC ) Size exclusion chromatography ( SEC ) Affinity chromatography Reverse phase chromatography ( RPC ) Normal phase chromatography Capillary electrophoresis ( CE ) and capillary electrochromatography ( CEC ) Multi‐dimensional separations New approaches Isotope‐coded affinity tag method ( ICAT ™) Multi‐epitope ligand‐Kartographie ( MELK ) Conclusion