Effect of Chinese herbal compound Tengmei decotion on IL-17/NF-κB signal pathway in synovium tissue of rat arthritis models induced by type II collage

Purpose: To investigate the biological effect of IL-17/NFB immune inflammatory pathway on pathological damage in synovium tissue of Rheumatoid arthritis (RA) and study in regulation mechanism of Chinese herbal compound Tengmei decotion for IL-17/NFB signal pathway. Methods: To establish collagen II-induced rats arthritis(CIA)models. The successful models of SD rats were randomly divided intomodel group, positive group, and high-, medium-groupsofChinesemedicine, 6 rats in eachgroup. The normal control and model groups were given distilled water (10mlkg-1d) by gavage. The positive drug group was given leflunomide (1.87gkg-1d) by gavage. The high and medium dose Chinese medicine groups were given crude medicine of 31.8 gkg-1d and 15.9 gkg-1d by gavage. After twelve weeks of treatment intervention, all SD rats were executed, the blood and synovium tissue samples were kept for detecting IL-17, NF-KB P65 mRNA transcription and protein expression by RT-PCR, WESTEN BLOT and ELISA Analysis, and detecting inflammatory infiltration in synovium tissue by Histopathological analysis. Results: (1)Compared to the normal control group, levels of mRNA transcription and protein expression of IL-17 and NFB P65 were significantly up-regulated (P < 0.01) in the model group. Compared to the model group, levels of mRNA transcription and protein expression of IL-17 and NFB P65 were significantly down-regulated in the positive and Chinese medicine groups (P < 0.01).(2) Histopathological analysis displayed that mild hyperplasia of epithelial cells covering the articular cartilage synovium, joint cavity narrowing, and mild inflammatory lymphocytes infiltration in model group, with joint lesions improved in treatment groups. Conclusion: The molecular mechanisms of Chinese herbal Tengmei decotion in inhibiting immune inflammatory pathological damage in synovium of CIA rats models related to its effects on IL-17/NFB pathway. Contact: Yanan Wang, xingmiao2008@live.cn