Characterization of Bacillus sp. and Protease Production in SSF

Different types of enzymes are produced by bacteria, but proteases are the most widely used. In the present study, protease producing Bacillus sp. was isolated from dairy effluent and charecterized. The isolate could use all carbohydrates tested and optimum pH, NaCl and temperature were 8.5, 2.25 and 37oC. Beef extract was most suitable carbon source. The isolate showed better growth in presence of Mg+2 and K+ salts but Fe+2 and Co+2 were most inhibitory. The isolate also produced amylase and CMCase but maximum yield was of protease after 48h in Smf, and in SSF 125 U protease/g biomass was pro- duced in 1:9 proportion of cotton seed cake and rice husk.