Extracellular compounds from marine bacteria Shewanella sp. with high biotechnological potential.

Event Abstract Back to Event Extracellular compounds from marine bacteria Shewanella sp. with high biotechnological potential. Carina Marques#1, Susete Pinteus#1*, André Horta1, Nádia S. Fino1, Celso Alves1, Joana Silva1 and Rui Pedrosa1* 1 School of Tourism and Maritime Technology - IPL, Portugal Recently, marine bacteria have received great attention for the search of biologically active substances. As they are continuously competing for space and nutrients they produce a variety of secondary metabolites which inhibit the settlement of potential competitors such as fouling bacteria, invertebrates or larvae. This harsh environment plays a crucial role in the evolution of marine microorganisms making them excellent candidates for the search of bioactive compounds. In this work, the extracellular compounds of Shewanella sp., recently identified as Bifurcaria bifurcata epiphytic bacteria, were evaluated for their antimicrobial and antioxidant potential. Shewanella sp. was grown in Marine Broth during three days in which point the bacteria was centrifuged and the supernatant collected and concentrated for further screening. The antimicrobial potential was evaluated against four model microorganisms: Escherichia coli (ATCC 25922); Staphylococcus aureus (ATCC 25923); Saccharomyces cerevisiae (ATCC 9763) and Bacillus subtilis (ATCC 6633). The ability of extracellular compounds to inhibit microorganism’s growth was accompanied by optical density at 600 nm and the results were expressed as percentage of control. All the tests were performed at 1 mg/ml. The antioxidant activity was evaluated by both, the quantification of the total phenolic content (TPC) and by the evaluation of the DPPH radical scavenging activity. i>Shewanella sp. revealed to produce compounds with high antimicrobial activity against all studied microorganisms, namely with 63.8±3.36% of inhibition against B. subtilis; 60.9±8.64% against S. cerevisiae; 50.66±6.7% against S. aureus and 41.86±2.33% against E. coli. Regarding the antioxidant activity, the supernatant reveal to contain compounds with some ability to scavenge the DPPH radical at 1mg/ml, with a reduction of 29.85±1.68% of control and a total phenolic content of 0.3 mg gallic acid equivalent/g of extract. The extracellular compounds of Shewanella sp. reveal to be a very promising source for antimicrobial compounds that can have further biotechnological application; moreover the extraction method offers important advantages: is simple, sustainable and environmentally friendly. #Carina Marques and Susete Pinteus contributed equally to this work. Keywords: marine microorganisms, antimicrobial compounds, antioxidant activity, Epiphytic bacteria, Marine natural compounds Conference: IMMR | International Meeting on Marine Research 2014, Peniche, Portugal, 10 Jul - 11 Jul, 2014. Presentation Type: Poster Presentation Topic: BLUE BIOTECH Citation: Marques# C, Pinteus# S, Horta A, Fino NS, Alves C, Silva J and Pedrosa R (2014). Extracellular compounds from marine bacteria Shewanella sp. with high biotechnological potential.. Front. Mar. Sci. Conference Abstract: IMMR | International Meeting on Marine Research 2014. doi: 10.3389/conf.fmars.2014.02.00072 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 12 May 2014; Published Online: 18 Jul 2014. * Correspondence: Dr. Susete Pinteus#, School of Tourism and Maritime Technology - IPL, Peniche, 2520–641, Portugal, susete.pinteus@ipleiria.pt Prof. Rui Pedrosa, School of Tourism and Maritime Technology - IPL, Peniche, 2520–641, Portugal, rpedrosa@ipleiria.pt Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Carina Marques# Susete Pinteus# André Horta Nádia S Fino Celso Alves Joana Silva Rui Pedrosa Google Carina Marques# Susete Pinteus# André Horta Nádia S Fino Celso Alves Joana Silva Rui Pedrosa Google Scholar Carina Marques# Susete Pinteus# André Horta Nádia S Fino Celso Alves Joana Silva Rui Pedrosa PubMed Carina Marques# Susete Pinteus# André Horta Nádia S Fino Celso Alves Joana Silva Rui Pedrosa Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. 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