Nmr Quantitative Analysis Of Complex Mixtures

The intrinsic quantitative nature of NMR is increasingly exploited in areas ranging from complex mixture analysis (as in metabolomics and reaction monitoring) to quality assurance/control (QA/QC). One of the key advantages of NMR over other methods for quantitative analysis is that molar concentration can be determined directly from signal intensity, regardless of the chemical nature, and can be accomplished without requirement of reference substances. In this short review, we attempt to capture the various aspects of data collection and data analysis for NMR quantitative analysis of complex mixtures. While the requirements for quantitative data collection have been well recognized, the data analysis methodologies are fast growing. With significant increase in the computation speed, time-domain analysis (an alternative to FT) is emerging as potential alternative to frequency-domain analysis and poses a good opportunity for automatable processes for high-throughput NMR quantitation.