"Human anti-langerin (CD207) polyclonal antibodies detect epidermal Langerhans-like cells in the sea turtle (Chelonia mydas)"

Event Abstract Back to Event "Human anti-langerin (CD207) polyclonal antibodies detect epidermal Langerhans-like cells in the sea turtle (Chelonia mydas)" Fernando A. Muñoz1*, Juana Calderon-Amador2, Ana Negrete-Philippe3 and Leopoldo Flores-Romo2 1 Universidad Autónoma de San Luis Potosí, Mexico 2 CINVESTAV, Mexico 3 Parque Xcaret, Tortugas marinas, Mexico Dendritic cells form the link between the innate and adaptative immune response, particularly on mucosal and epidermal surfaces. The Langerhans cells play a key role in the skin immune response, however, no molecular marker for these cells has been identified in endangered marine reptile green turtles thereby complicating the understanding of the pathogenesis of diseases such as fibropapillomatosis which induces internal and skin tumors in this species worldwide. The Langerhans cells present different molecular markers, among them, HLA-DR, MHC-II, CD1A, and specially (CD207). We demonstrate that the polyclonal anti-human langerin (CD207) antibodies strongly stained a langerine-like cell in the suprabasal layer of epidermis in cryosections and cytospin preparation from migration assay. The morphology of these cells was round to amoeboid in normal skin and cytospin preparation, but in skin with ulcerative dermatitis, langerine+ cells aggregated around ulcers and adopted a more pleomorphic morphology. To our knowledge, this is the first identification of Langerhans-like cells with a molecular marker in a reptile species. References Nishibu A., Ward B.R., Jester J.V., Ploegh H.L.,Boes M., Takashima A.(2006). 143 Behavioral Responses of Epidermal Langerhans Cells In Situ to Local Pathological Stimuli. 144 J Invest Der 126, 787–796 Baker, K.W., Habowsky, J.E., (1983). EDTA separation and ATPase langerhans cell staining in the mouse epidermis. J. Invest. Dermatol. 80, 104–107. Bursch L.S. , Wang L., Igyarto B., Kissenpfennig A., Malissen B., Kaplan D.H., Hogquist 150 K.A. Identification of a novel population of Langerin + dendritic cells. J Exp Med. 2007. 151 204:3147-56. Clausen B.E., Kel J.M. (2010). Langerhans cells: critical regulators of skin immunity?. 155 Immunol. cell Biol. 88, 351–360 Carrillo-Farga, J., Perez, T.A., Castell, R.A., Antuna, B.S. (1991). Adenosine 157 triphosphatase-positive langerhans-like cells in the epidermis of the chicken (Gallus 158 gallus). J. Anat. 176, 1–8. Chaloupka M., Balazs G.H., Work, T.M.(2009) Rise and Fall over 26 Years of a Marine 161 Epizootic in Hawaiian Green Sea Turtles. J. Wildlife Dis. 45:1138-1142 Ginhoux, F., Merad, M. (2010). Ontogeny and homeostasis of Langerhans cells. Immunol. 164 Cell. Biol. 88:387-392 Henri, S., Guilliams M.,,Poulin L.M., Tamoutounour, S., Ardouin L., Dalod M., Malissen, B. 167 (2010). Disentangling the complexity of the skin dendritic cell network. Immunol. Cell. Biol. 168 88:366-375. Lovly J., Savidant G.P,, Speare D.J., Wright G.M.(2009). Langerin/CD207 positive 171 dendritic-like cells in the haemopoietic tissues of salmonids. Fish Shellfish immunol. 172 27:365-368. Keywords: Langerhans Cells, Chelonia mydas, CD207, Sea Turtle, anti-langerine Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Innate immunity Citation: Muñoz FA, Calderon-Amador J, Negrete-Philippe A and Flores-Romo L (2013). "Human anti-langerin (CD207) polyclonal antibodies detect epidermal Langerhans-like cells in the sea turtle (Chelonia mydas)". Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00871 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 19 Jun 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. Fernando A Muñoz, Universidad Autónoma de San Luis Potosí, Mexico City, Mexico, fernando.munoz@uaslp.mx Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Fernando A Muñoz Juana Calderon-Amador Ana Negrete-Philippe Leopoldo Flores-Romo Google Fernando A Muñoz Juana Calderon-Amador Ana Negrete-Philippe Leopoldo Flores-Romo Google Scholar Fernando A Muñoz Juana Calderon-Amador Ana Negrete-Philippe Leopoldo Flores-Romo PubMed Fernando A Muñoz Juana Calderon-Amador Ana Negrete-Philippe Leopoldo Flores-Romo Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.