SSR标记鉴定甜瓜品种‘红月亮’种子纯度
China Cucurbits and Vegetables(2014)
Abstract
为明确甜瓜品种‘红月亮’F1代的种子纯度,通过提取甜瓜‘红月亮’子叶DNA,利用SSR (Simple Sequence Repeats)分子标记对甜瓜品种红月亮F1代种子DNA进行了PCR扩增、检测与分析.结果表明,在20个SSR引物组合对亲本及F1代筛选中,共有6对引物组合具有多态性,多态率30%.选择CMBR026用于‘红月亮’F1代种子纯度鉴定,使用该标记共检测118株,其中2株为自交株,种子纯度为98.3%;试验用的‘红月亮’种子田间鉴定纯度为99.5%,鉴定结果一致.SSR分子标记方法更能准确鉴别出‘红月亮’中的不纯株,且大大缩短纯度鉴定周期.
MoreKey words
Melon,Red Moon,SSR,Purity test
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