Study on Extraction of Polysaccharide by Composite Enzymatic Dilapidating Walls from Waste Wine Yeast Slurry

Objective: To establish a method for the extraction of polysaccharide from waste wine yeast slurry by composite enzymatic method. Methods: On the basis of single factor experiments, the effects of operating conditions, such as enzyme mixture ratio, enzyme added, composite enzymatic extraction pH, and composite enzymatic extraction temperature, were analyzed by orthogonal design methodology. Results: The optimized conditions of composite enzymatic extraction are as follows: the ratio of beta-glucan enzyme to papain enzyme is 3:1, composite enzyme concentration 2.5 g/L, pH value 6.0, temperature 60 degrees C, the polysaccharide yield is 8.97%. Conclusion: The composite enzymatic extraction method has a high extraction rate in extracting the polysaccharide of waste wine yeast slurry. The method is simple and practical.