Quantitative Assessments Of Glycolysis From Single Cells

The most common positron emission tomography (PET) radio-labeled probe for molecular diagnostics in patient care and research is the glucose analog, 2-deoxy-2-[F-18] fluoro-D-glucose (F-18-FDG). We report on an integrated microfluidics-chip/beta particle imaging system for in vitro F-18-FDG radioassays of glycolysis with single cell resolution. We investigated the kinetic responses of single glioblastoma cancer cells to targeted inhibitors of receptor tyrosine kinase signaling. Further, we find a weak positive correlation between cell size and rate of glycolysis.