Research regarding haemocyte profile from Apis mellifera carpatica bee haemolymph originated in the south of Romania.

The studies were carried out in 2007-2008, in the frame of the Pathology Department from Beekeeping Research and Development Institute Bucharest. There were performed morpho-cytological investigations on haemolymph samples collected from honeybees originated from honeybee colonies located in the southern part of Romania. These researches mainly aimed to identify and classify the haemocytes according to the literature, to study the morphological characters of haemocytes, to establish an optimal staining method for morphologic studies, to study the stage transformation of haemocytes. The haemolymph samples were collected from healthy honeybees and slides were prepared using the following staining methods: Giemsa, May Grunwald-Giemsa and May Grunwald-Giemsa (according to Shapiro), modified and adapted for the haemocytes sensitivity testing. The following parameters of the haemocytes from centrifuged and non-centrifuged haemolymph were investigated and described: the density and their type, percentage of the different cell types in honeybees from healthy and natural infected colonies, stages of haemocytes formation by morphometrical analyses using specific measurement software.