Vast differences in the levels of host markers detected in saliva and serum and their potential for diagnosing tb disease

Event Abstract Back to Event VAST DIFFERENCES IN THE LEVELS OF HOST MARKERS DETECTED IN SALIVA AND SERUM AND THEIR POTENTIAL FOR DIAGNOSING TB DISEASE Khutso G. Phalane1*, Gerhard Walzl1*, Magdalena Kriel1, Andre G. Loxton1, Angela Menezes1, Kim Stanley1, Gian Van Der Spuy1 and Novel Chegou1* 1 University of Stellenbosch, Department of Biomedical Sciences, South Africa Background Despite the recent introduction of molecular beacon assays such as the GeneXpert into clinical practice, the diagnosis of tuberculosis (TB) disease remains challenging in individuals with difficulty in providing good quality sputum samples such as children. Host biosignatures of inflammatory markers could be valuable in such cases, especially if they are based on more easily obtainable samples such as saliva, and are developed into rapid, point-of-care tests. The aim of this study was to compare the levels of host markers detectable in the saliva and serum of patients with possible pulmonary TB, and to assess the diagnostic utility of these markers for TB disease. Methods Saliva and serum samples were collected from 38 TB suspects who were recruited from a community health centre in Cape Town, after which the levels of 33 host markers were evaluated in the samples using the Luminex platform. Results Of the 38 TB suspects enrolled, activeTB disease was confirmed in 11(28.9%) by sputum culture. In both the TB cases and non-cases, the levels of most markers were above the minimum detectable limit in both sample types, but marker levels were not consistently higher in one sample type. The levels of fractalkine, IL-17, IL-6, IL-9, MIP-1β, CRP, VEGF and IL-5 in saliva, and those of IL-6, IL-2, SAP and SAA in serum, were significantly higher in TB patients, in comparison to the levels obtained in those without active TB (p<0.05). The area under the ROC curve was ≥ 0.70 for most of these markers, thereby confirming their diagnostic potential for TB disease. Conclusions There are vast differences in the levels of host markers expressed in saliva in comparison to serum and some markers in both sample types have potential in the diagnosis of TB disease. These preliminary findings warrant further investigation. Acknowledgements Funding: This work was funded by the EDCTP through the African European Tuberculosis Consortium (AE-TBC, grant number IP_2009_32040) and the Trials of Excellence in Southern Africa (TESA, project code CG_cb_07_41700). Keywords: Cytokines, diagnosis, Saliva, Serum, Tuberculosis Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Innate immunity Citation: Phalane KG, Walzl G, Kriel M, Loxton A, Menezes A, Stanley K, Van Der Spuy G and Chegou N (2013). VAST DIFFERENCES IN THE LEVELS OF HOST MARKERS DETECTED IN SALIVA AND SERUM AND THEIR POTENTIAL FOR DIAGNOSING TB DISEASE. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00638 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 12 Jun 2013; Published Online: 22 Aug 2013. * Correspondence: Miss. Khutso G Phalane, University of Stellenbosch, Department of Biomedical Sciences, cape town, Western Cape, South Africa, khutsokido@gmail.com Prof. Gerhard Walzl, University of Stellenbosch, Department of Biomedical Sciences, cape town, Western Cape, South Africa, gwalzl@sun.ac.za Dr. Novel Chegou, University of Stellenbosch, Department of Biomedical Sciences, cape town, Western Cape, South Africa, novel@sun.ac.za Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Khutso G Phalane Gerhard Walzl Magdalena Kriel Andre G. Loxton Angela Menezes Kim Stanley Gian Van Der Spuy Novel Chegou Google Khutso G Phalane Gerhard Walzl Magdalena Kriel Andre G. Loxton Angela Menezes Kim Stanley Gian Van Der Spuy Novel Chegou Google Scholar Khutso G Phalane Gerhard Walzl Magdalena Kriel Andre G. Loxton Angela Menezes Kim Stanley Gian Van Der Spuy Novel Chegou PubMed Khutso G Phalane Gerhard Walzl Magdalena Kriel Andre G. Loxton Angela Menezes Kim Stanley Gian Van Der Spuy Novel Chegou Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.