AB194. Adipose derived stem cell differentiated towards urothelium phenotype both in vivo and in vitro: a potential use for bladder defect regeneration

Experimental ASCs were isolated from the human adipose tissue of individuals undergoing liposuction procedures. Labeling with CM-DiI, the ASCs were mixed with an immortalized urothelium cell line (HUC) and implanted into the subcutaneous tissue of athymic mice for 4 weeks. In vitro, ASCs were induced by conditioned media (CM) and the transwell co-culture with HUC for 21 days. Protein and mRNA expression of the urothelium specific markers UP-1A and UP-II were detected. Array detection of the upper medium was used to screen 41 cytokines and receptors in induced ASCs at different time points.