Delivery of human EV71 receptors by adeno-associated virus increase EV71 infection in adult mice

Event Abstract Back to Event Delivery of human EV71 receptors by adeno-associated virus increase EV71 infection in adult mice Shih-Jen Liu1, 2*, Hon-Ber Shiao1, Ai-Hsiang Chou1, Su-I Lin1, I-Hua Chen1, Chia-Chyi Liu1, Pele Chong1 and Mi-Hua Tao3 1 National Health Research Institutes, Taiwan 2 China Medical University, Taiwan 3 Academia Sinica, Taiwan Enterovirus 71 (EV71) is responsible for hand, foot, and mouth disease in children under 6 years of age. Recently, there are two EV71 receptors in human have been identified human P-selectin glycoprotein ligand-1 (hPSGL-1) and human scavenger receptor class B, member 2 (hSCARB2) as the functional EV71 receptors. We hypothesized that delivery of hPSGL-1 by adeno-associated virus (AAV) could increase EV71 infection in adult mice. To prove this hypothesis, both green hSCARB2 and hPSGL-1 gene was cloned into AAV vector to generate rAAV- hSCARB2 and rAAV-hPSGL-1 virus. We found that oral and intravenous administration of rAAV-GFP can express highest level of GFP in the intestine and other organs. Based on this result, rAAV-hSCARB2 and rAAV-hPSGL-1 were administered by oral and intravenous route for gene transfer. The expression levels of both hSCARB2 and hPSGL-1 in the intestine and brain were analyzed using real-time PCR and Immunohistochemistry. Histopathological examination revealed that the expression of hSCARB2 and hPSGL-1 in brain and intestine were detected after 4 weeks. After EV 71 infections, we also found that both hSCARB2 and rAAV-hPSGL-1 infected mice could up-regulate cytokines secretion in brain or intestine. Moreover, the infectious EV71 viral particles were detected in the brain and intestine from the infected animals. Although the increased EV71 infection did not show neurological manifestations, this technology could be applied to innate receptor-deficient mice to increase their infection and pathogenesis in adult mice. Acknowledgements Grant support: This work was supported by the National Council grant NSC 100-2325-B-400 -015 – Keywords: Enterovirus, EV71 receptor, adeno-associated virus, animol model, EV71 Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Host-pathogen interactions Citation: Liu S, Shiao H, Chou A, Lin S, Chen I, Liu C, Chong P and Tao M (2013). Delivery of human EV71 receptors by adeno-associated virus increase EV71 infection in adult mice. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00219 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 12 Mar 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. Shih-Jen Liu, National Health Research Institutes, Miaoli County, Taiwan, levent@nhri.org.tw Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Shih-Jen Liu Hon-Ber Shiao Ai-Hsiang Chou Su-I Lin I-Hua Chen Chia-Chyi Liu Pele Chong Mi-Hua Tao Google Shih-Jen Liu Hon-Ber Shiao Ai-Hsiang Chou Su-I Lin I-Hua Chen Chia-Chyi Liu Pele Chong Mi-Hua Tao Google Scholar Shih-Jen Liu Hon-Ber Shiao Ai-Hsiang Chou Su-I Lin I-Hua Chen Chia-Chyi Liu Pele Chong Mi-Hua Tao PubMed Shih-Jen Liu Hon-Ber Shiao Ai-Hsiang Chou Su-I Lin I-Hua Chen Chia-Chyi Liu Pele Chong Mi-Hua Tao Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.