Food intake, digestive enzyme activity and contraction of the gut is regulated by sulfakinins from the cricket Gryllus bimaculatus

Event Abstract Back to Event Food intake, digestive enzyme activity and contraction of the gut is regulated by sulfakinins from the cricket Gryllus bimaculatus Martina Meyering-Vos1*, Sandra Diersch1, Reinhard Predel2 and Joseph Woodring1 1 University of Bayreuth, Animal Ecology I, Germany 2 University of Jena, Institute of Zoology, Germany In vertebrates, a variety of mechanisms are involved in the regulation of food intake and energy balance, and recent developments in insect research indicate that the endocrine regulation of these processes in invertebrates is at least as complex. Sulfakinins (SK) are neuropeptides structurally displaying sequence homology with the hormonally active portion of the vertebrate gastrin/CCK peptide family. In Gryllus bimaculatus two sulfakinins (I, II) are encoded on the gene and the peptides could be identified in brain extracts from adult females by mass spectrometry analysis. In Gryllus bimaculatus after in vivo degradation of the specific sulfakinin mRNAs by the RNA interference method, the expression is reduced and this leads to a drastic increase in the food-intake indicating the inhibitory bioactivity of the peptides. Synthetic SK I peptide has a stimulatory effect on the release of α-amylase activity in the ventriculus, whereas SK II stimulates the release of this enzyme in the caecum of unfed crickets. Additionally, the sulfakinins stimulate the release of proteases into the caecum. SK I stimulates trypsin release and in a similar manner as gastrin stimulates biosynthesis in vertebrates. SK II increases most specifically the released aminopeptidase activity. Finally, SK I stimulates the myoactivity of the crop, wheras SK II has no effect. Acknowledgements This work was supported by the DFG Me 2034/1-3. Keywords: amylase, food intake regulation, Gastrointestinal System, gut myoactivity, Neuropeptide, Protease, satiety, Sulfakinin Conference: 25th Conference of the European Comparative Endocrinologists, Pécs, Hungary, 31 Aug - 4 Sep, 2010. Presentation Type: Conference Presentation Topic: Comparative endocrinology Citation: Meyering-Vos M, Diersch S, Predel R and Woodring J (2010). Food intake, digestive enzyme activity and contraction of the gut is regulated by sulfakinins from the cricket Gryllus bimaculatus. Front. Endocrinol. Conference Abstract: 25th Conference of the European Comparative Endocrinologists. doi: 10.3389/conf.fendo.2010.01.00081 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 10 Sep 2010; Published Online: 13 Sep 2010. * Correspondence: Dr. Martina Meyering-Vos, University of Bayreuth, Animal Ecology I, Bayreuth, Germany, martina.meyering-vos@uni-bayreuth.de Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Martina Meyering-Vos Sandra Diersch Reinhard Predel Joseph Woodring Google Martina Meyering-Vos Sandra Diersch Reinhard Predel Joseph Woodring Google Scholar Martina Meyering-Vos Sandra Diersch Reinhard Predel Joseph Woodring PubMed Martina Meyering-Vos Sandra Diersch Reinhard Predel Joseph Woodring Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.