Synthesis and Inhibitory Activity of Novel Ferrocenyl 1,2,4-Triazole Mannich Bases

15 novel 3-ferrocenyl-1,2,4-triazole Mannich bases (3a similar to 3e, 4a similar to 4e and 5a similar to 5e) were designed and synthesized for the first time by Mannich reaction from five 3-ferrocenyl-1,2,4-triazole Schiff bases, which reacted with morpholine, 1-methylpiperazine and piperazine, respectively. The structures of 3, 4 and 5 were characterized by IR, H-1 NMR and HRMS. The X-ray single crystal diffraction of 3b was obtained. The results showed that the target compounds were successfully synthesized. The target products were evaluated for their Cdc25B inhibitory activities. The test results indicated that 13 of 15 Mannich bases exhibited good inhibitory activity, wherein IC50 values of 3c, 4a, 5b, 5c and 5e were lower than the positive control compound Na3VO4 (1.86 +/- 0.24 mu g.mL(-1)) with 0.91 +/- 0.18, 0.62 +/- 0.13, 1.10 +/- 0.33, 0.44 +/- 0.04 and 0.67 +/- 0.13 mu g.mL(-1), respectively. Compared with PTP1B inhibition activity results, the target products have a selective inhibitory effect on Cdc25B.