The differences in expression levels of depression-related proteins in hippocampal of CUMS rats by treating of electro acupuncture

Purpose: The meridian system is originated from concept of invisible line which connects between internal organ and specific point in the body surface. And specific points are discovered by observing reaction of patients after pushing patient’s skin with doctor’s hand. And it is so called Ashipoints which means points make patient scream “Ah!”. In this study, we suppose that the ashi-points have relation with referred pain, and thus observed an aspect of revelation of referred pain area visualized by evans blue dye. Methods: SD-rats (250 g) were injected evans blue dye through caudal veinwhile anesthetizedwithmixture of Zoletil and Rom pun. 10minutes later, silicone guide and Q-tip absorbing mustard oil was inserted to the large intestine in depth of 3, 5. 7 cm from the anus. Evans blue signwas observed after 30minutes after insert of theQ-tip. The location, size and shape of evans blue sign was recorded in the chart developed to record the sign and charts weremerged by u sing the Adobe Photoshop program. Results: More than 90% of evans blue sign were located in the hind paw. Specially, many signs were located in the lateral side of the foot (border between the red and white flesh). Most of signs have shape of long ellipse, and headed samedirection. The sign mainly located in rows around navicular tubercle. The signshad tendencyof distinguishedby thedepthbut there were no significant border for distinguish the signs. Conclusion: In this study, we have visualized referred pain area by using evans blue dye, and confirmed that the signs has tendency of forming lines, and many acupuncture points are located in the same anatomical area. In spite of the results of this study, we have confirmed only one-way communication and to confirm two-way communication, we plan to ob serve treatment effects of the sign area. Contact: Osang KWON, logos319@kiom.re.kr