High-level production of replication-defective human immunodeficiency type 1 virus vector particles using helper-dependent adenovirus vectors

A rotary union assembly includes a rotor with a housing rotatably mounted on a forward end thereof, the rotor being selectively releasably mounted in one end of a tubular support member. The rotor includes a socket extending radially from a central shaft and a spring loaded plunger within the socket having a pin cooperable with a hole in the tubular member side wall to releasably secure the rotor in position in the tubular member. A hose barb on the rearward end of the rotor shaft permits easy installation of a resilient, flexible hose on the rotor.