An Evaluation of Automated fluorescent PCR-Based DNA Typing

The recent revolution of new technologies in forensic DNA typing has presented several promising PCR-based identification systems. Compared to current RFLP VNTR systems, PCR offers the advantages of enhanced sensitivity, more discrete alleles and the efficacy of working with degraded DNA samples. In order to achieve the equivalent DNA discrimination experienced with RFLP VNTR analysis, the new PCR-based systems will require a greater number of STR systems to be run or additional procedures involving AmpFLPs systems or digital DNA typing (MVR). A concerted approach using several DNA typing technologies represents the future reality in the processing of numerous biological forensic samples. PCR-based DNA typing with automated real-time analysis of fluorescent amplification products is currently a major thrust of the RCMP DNA program.