Molecular Comparison Of The Genetic Identity Of Maize Genotypes With Different Age

Based on year of seed production, 1994, 1996 and 2000, this study compared moleculsrly four maize endogamic lines generated by the INIEAP Maize Program: Ll, L2, L3 and L4. DNA was extracted from seed embryos and subjected to PAPD protocols, after which phylogenetic relations were established between treatments using the program LCDMV (molecular distance between varieties calculation software), using the parameters: Scud distance = 0, 95% confidence, the estimator of distances of Nei and Li, and the method of average clustering (UPGMA). In the comparison by age, the dendro-graph obtained grouped equal genotypes according to their respective age, with outstanding similitude coefficient reached by the unon of ages 1,2 (90%) and L4 (80%), while the similarity value for Ll was 70%. The genotipic capacity of the lines to preserve their genome was higher in L2, followed by L4 and Ll. The results indicated the effectiveness of the RAPD protocol to discriminate within and between genetically-related genotypes according to age, and to distinguish within and between maize lines irrespective of their age.