In vitro inhibitory effect of the hydro-alcoholic extract from the avicennia marina (hara) on candida albicans

Introduction: Candida albicans is one the most common opportunistic fungal diseases in humans. For a long time and even now the leaf of the Avicennia marina (hara) herb has been used to treat many infections in southern parts of Iran. The purpose of this study is to determine the inhibitory effects of the hydro alcoholic extract of the Avicennia marina leaf on candida albicans under in vitro conditions. Methods: the Avicennia marina leaf was collected from the southern shores of Iran and after being washed and dried the extraction of the hydro alcoholic extract was carried out. The effect of the extract was applied using the disk diffusion method and diffusion in agar by creating soak away pits (well) in different concentrations. The amount of MIC and MFC was determined by Macrobroth dilution method. The data analysis was done using the SPSS (version 18) software and the repeat measure test. Results: In the disk diffusion method in 80 μg/disk concentration the inhibition zone was equal to 18.7mm and in the agar well diffusion method in the 80 mg/ml concentration the inhibition zone was equal to 20mm. The values for MIC and MFC for the clinical samples were 55 and 70 μg/disk consecutively. Conclusion: hydro alcoholic extract of avicennia marina has significant effect on candida albicans in vitro, thus, Recommended that further studies be done about this extract under In vivo conditions.