Wavelet domain mutual information synchronization of multimodal cardiac microscopy image sequences
Proceedings of SPIE(2009)
摘要
Multi-modal microscopy, such as combined bright-fiel and multi-color fluorescenc imaging, allows capturing a sample's
anatomical structure, cell dynamics, and molecular activity in distinct imaging channels. However, only a limited number
of channels can be acquired simultaneously and acquiring each channel sequentially at every time-point drastically reduces
the achievable frame rate. Multi-modal imaging of rapidly moving objects (such as the beating embryonic heart), which
requires high frame-rates, has therefore remained a challenge. We have developed a method to temporally register multimodal,
high-speed image sequences of the beating heart that were sequentially acquired. Here we describe how maximizing
the mutual information of time-shifted wavelet coefficien sequences leads to an implementation that is both accurate and
fast. Specificall , we validate our technique on synthetically generated image sequences and show its effectiveness on
experimental bright-fiel and fluorescenc image sequences of the beating embryonic zebrafis heart. This method opens
the prospect of cardiac imaging in multiple channels at high speed without the need for multiple physical detectors.
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关键词
sensors,microscopy,heart,mutual information,wavelets
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