Effect of different extenders on ram sperm traits during storage

HEGEDŰSOVA, Z., STOLC, L., LOUDA, F., CUNAT, L., VEJNAR, J.: Eff ect of diff erent extenders on ram sperm traits during storage. Acta univ. agric. et silvic. Mendel. Brun., 2012, LX, No. 6, pp. 111-116 The aim of the study was to test commercial extenders used for short-term and long-term sperm preservation. Semen was collected in the reproduction season, i.e. from June to December. The ejaculates were obtained from single services and the routine analysis of the semen was performed immediately aer the collection. The examination included semen volume, colour and texture, sperm concentration and motility, ejaculate turbulence and percentage of sperm with abnormal morphology. The semen was diluted with an extender in the ratio of 1:4. The processed semen was transported in an insulated container at 16-18 °C to the laboratory and stored in a stationary thermostat under the same temperature. Sperm motility tests were performed 24, 48, 72 and 96 hours aer the placement in to thermostat. Ejaculates diluted with Ovipro, Optidyl, Triladyl and Andromed CSS gave very good results of viability (81.23 %-83.41 %) aer 24 hours of storage. Aer 48 hours, Ovipro, Andromed, Optidyl and Triladyl gave values above 75 %. The Triladyl extender proved to be a good stabilizing agent, showing consistent results during a long-term storage. It was chosen as a control one for overall assessment. Other preservation media did not show any improving or worsening eff ects. The extender Ovipro showed a high motility eff ect in the fi rst 48 hours only, and hence it appears to be the best solution for the short-term preservation. ram, sperm activity, extender