Intravitalmikroskopische Charakterisierung der frühen Vaskularisierung und Angiogenese-Hemmung des Pankreaskarzinoms im Rückenhautkammer-Modell

Objective: Vessel sprouting is a prerequisite for tumor growth. The aim of this study was to characterize the early vascularization of pancreatic cancer by intravital microscopy and to evaluate the effects of an angiogenesis inhibitor. Materials and methods: Donor tumors of the ductal pancreatic cancer cell line DSL-6A were implanted into a dorsal skin fold chamber of Lewis rats. The therapy group (n = 8) received TNP-470 and the controls (n = 8) a carrier solution. The animals were examined daily by intravital microscopy until day 7. Results: The number of newly formed tumor vessels in the therapy group on day 7 was 6.0, the vessel density 17.4/cm and the tumor size 0.79 qmm. In the control group the number of newly formed tumor vessels averaged 9.2, the vessel density 54.0/cm and the tumor size 1.43 qmm. Conclusion: The results show the effectiveness of an angiogenesis inhibitor in the early phase of tumor vascularization and the suitability of the dorsal skin fold chamber model for direct and continuous observation of tumor vascularization.