Sysmex CA-1500全自动血凝仪PT-der法和Von-Clauss法检测纤维蛋白原的分析
International Journal of Laboratory Medicine(2014)
Abstract
目的:评价Sysmex CA-1500全自动血凝仪PT-der法和Von-Clauss法测定血浆纤维蛋白原(Fib)的相关性和准确性。方法采用Sysmex CA-1500全自动血凝仪PT-der法和Von-Clauss法分别测定755例血液标本的血浆Fib浓度;高Fib浓度标本的稀释比例为1∶8、2∶7、3∶6、4∶5、5∶4、6∶3,以稀释比例为横坐标,以2种方法测得的Fib浓度为纵坐标,进行简单线性回归分析。结果 Fib:2.0~<6.0 g/L时,Von-Clauss法检测Fib值高于PT-der法(P<0.01);Fib:<2.0 g/L ,>6.0 g/L时, PT-der法检测Fib值高于Von-Clauss法(P<0.01)。PT-der法和Von-Clauss法检测的线性回归方程分别为:Y =4.5377 X+1.5513(R2=0.8973),Y=7.7922X+0.2900(R2=0.9805)。结论 Von-Clauss法能较好地反映人体内具有凝血功能的Fib水平。
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Key words
fibrinogen,Von-Clauss assay,PT-der assay,linear regression
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