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间期荧光原位杂交技术检测骨髓增生异常综合征-7/7q-染色体异常的价值

Sheng-hua JIANG,Hong LIU,Li YANG,Zeng-hua LIN,Xin CAO,Wei LU,Guo-qi SONG,Zuo-feng DING,Ya-qing TANG,Jian-hong LU

SHANDONG MEDICAL JOURNAL（2010）

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Abstract
目的 探讨应用间期荧光原位杂交(FISH)技术检测7号染色体/7号染色体长臂缺失(-7/7q-)异常在骨髓增生异常综合征(MDS)中的价值.方法 采用常规细胞遗传学(CC)和间期FISH检测技术对72例MDS患者和8例正常对照者的骨髓细胞进行分析,比较CC和FISH检测-7/7q-异常的情况.结果 72例MDS中,12例CC检测阳性,发生率16.7%.间期FISH检测24例阳性,发生率33.3%.两种方法检出率有统计学差异(P＜0.05).结论 间期FISH技术敏感性高于CC,并能发现和纠正CC分析中的漏检异常.
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Key words
myelodysplastic syndromes,fluorescence,situ hybridization,chromosome disorders
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