Apo E基因multi-ARMS与PCR-RFLP分型法的比较研究
mag(2002)
Abstract
目的:以聚合酶链反应-限制性酶切片段长度多态性(PCR-RFLP)方法作为参照,建立一种ApoE基因分型方法.方法:以基因组DNA为模板,用PCR-RFLP法和等位基因特异性复合PCR方法(Multiplex Amplification Refractory Mutation System,multi-ARMS)检测了75例个体的ApoE基因型.结果:共检测出5种常见的ApoE基因型:ε2/2,ε3/3,ε2/3,ε3/4,ε2/4.两种方法所得的结果完全一致.结论:说明multi-ARMS方法快速简便、准确可靠,具有推广应用价值.
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