Tegumental Changes in Adult Schistosoma mansoni Induced by a New Imidazolidinic Derivative

Aims: Verify the potential of the schistosomicidal imidazolidine derivative (5Z)-3-(4bromo-benzyl)-5-(4-chloro-benzylidene)-4-thioxo-imidazolidin-2-one.Study Design: In this study, we tested the imidazolidinic derivative 3 through in vitro evaluations, cytotoxicity assay and analysis of Scanning Electron Microscopy to verify its therapeutic potential in the treatment of schistosomiasis.Place and Duration of Study: Departamento de Antibioticos, Universidade Federal de Pernambuco (UFPE), Funda double dagger ao Oswaldo Cruz (FIOCRUZ)/PE and (FIOCRUZ)/BA between January 2013 and march 2014.Methodology: This study was approved by the Ethics Committee on Animal Use Research Center Aggeu Magalhaes/Oswaldo Cruz Fundaao (CPqAM/FIOCRUZ) authorized by the license No. 21/2011. Male albino Swiss mice were used Mus musculus 25 days old weighing 50 grams. Compound 3 was assayed for its cytotoxicity through cell J774 macrophage lineage. The amount of inhibitory concentration (LC50) was determined by nonlinear regression using the Graph Pad Prism version 5.01. Then the compound was evaluated against adult worms of S. mansoni by performing the activity in vitro at doses 100-20 mu g/mL and ultrastructural investigation by Scanning Electron Microscopy (SEM) at doses of 100 and 60 mu g/ml. The PZQ was the positive control of the experiment.Conclusion: The derivative imidazolidine 3 showed a promising schistosomicidal activity in vitro. However, conducting further studies with the completion of work in front of the live schistosomiasis is required.