Recent Advance on the Methods for Rapid Detection of Foodborne Pathogens

Rapid detection and identification of foodborne pathogens is an important prerequisite for timely and effective control and prevention of pathogen spread and food poisoning. Culture-based methods are too time-consuming for high-throughput testing and too tedious for analysis of samples with multiple organisms and provide little clinical information regarding the pathogen. With continued development of novel molecular-based technologies for rapid, high-throughput detection of foodborne pathogenic bacteria, a number of unique approaches and variations on existing techniques are currently on the market or are being implemented that offer ease of use, reliability and low cost. In this paper, the most frequently used assays for foodborne pathogens, including immunomagnetic separation, DNA-hybridization, capillary zone electromigration, conventional PCR, multiplex PCR, real-time PCR, DNA microarray, LAMP and biosensors were reviewed.