The role of gamma-delta T lymphocytes in the switch of Ig isotypes in B-1 cells under the influence of TI-2 antigens

Event Abstract Back to Event The role of gamma-delta T lymphocytes in the switch of Ig isotypes in B-1 cells under the influence of TI-2 antigens Nadezda Snegireva1*, Marina Gavrilova1 and Ekaterina Sidorova1 1 Mechnikov Institute of Vaccine and Sera of RAMS, Russia B-1 cells respond to T-independent antigens type 2 (TI-2 Ag) by formation of IgM antibodies/total Ig. At the same time in the small intestine B-1 cells may switch from IgM to IgA. Mechanism of the switch in the absence of T help is unknown. Now the role of gamma-delta T cells in the switch of Ig isotypes in B-1 cells under the influence of TI-2 Ags was studied. CBA mice were used. B-1 cells were isolated from peritoneal cavity, and intraepithelial gamma-delta T cells from small intestine. The purity of cell populations was determined by cytometry. Alpha (1→3) dextran (Dex) was used as TI-2 Ag. B-1 and gamma-delta T cells (10:1) were cultured in the round-bottom 96-well plates in RPMI 1640 medium with 10% of FCS and all necessary supplements in CO2-incubator for 4 days with or without Dex. The number of IgM- and IgA-forming cells were determined by ELISPOT method. It was found that: 1) the addition of Dex to peritoneal B-1 cells induced some increase in the numbers of IgM and IgA antibody- and Ig-forming cells (AFC and IFC, respectively); 2) the addition of gamma-delta T cells resulted in the decrease of the IgM-AFC and IgM-IFC number, and 3) the addition of gamma-delta T cells increased the quantities of IgA-AFC and IFC. The data obtained point to the possible role of intraepithelial gamma-delta T cells in the isotype switch in B-1 cells in response to TI-2 Ag. Keywords: gamma-delta T cells, B-1 cells, isotype switch, TI-2 antigens, immune response Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Adaptive Immunity Citation: Snegireva N, Gavrilova M and Sidorova E (2013). The role of gamma-delta T lymphocytes in the switch of Ig isotypes in B-1 cells under the influence of TI-2 antigens. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00198 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 11 Mar 2013; Published Online: 22 Aug 2013. * Correspondence: Miss. Nadezda Snegireva, Mechnikov Institute of Vaccine and Sera of RAMS, Moscow, Russia, hell59@mail.ru Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Nadezda Snegireva Marina Gavrilova Ekaterina Sidorova Google Nadezda Snegireva Marina Gavrilova Ekaterina Sidorova Google Scholar Nadezda Snegireva Marina Gavrilova Ekaterina Sidorova PubMed Nadezda Snegireva Marina Gavrilova Ekaterina Sidorova Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.