Identification of new S -RNase self-incompatibility alleles and characterization of natural mutations in Iranian almond cultivars
Trees(2012)
摘要
The two main objectives of this research were to identify new S -RNase alleles in Iranian almond cultivars and to characterize naturally occurring mutations in these alleles that may cause self-compatibility. We investigated S genotypes of 22 Iranian almond cultivars using stylar RNase electrophoresis, PCR and DNA sequencing. We report six previously unidentified P. dulcis S -RNase alleles ( S 45 , S 46 , S 47 , S 48 , S 49 and S 50 ). Four of 12 tested S -RNases were found to be non-functional in vitro: S 49 , S 50 , S 24 / S na and S 25 / S 47 . Detected point mutations in the C3 coding region of S 49 - and S 50 -RNase, leading to the replacement of a highly conserved cysteine and histidine residues, are with the highest probability the reason of these S -RNases inactivity. Results also suggested that ten Iranian almond cultivars display unique S genotype. All presented data confirm Iranian cultivars as valuable almond sources which are of interest to almond breeding and conservation programs.
更多查看译文
关键词
Almond,Prunus dulcis,Breeding,PCR,Self-incompatibility,S-allele
AI 理解论文
溯源树
样例
![](https://originalfileserver.aminer.cn/sys/aminer/pubs/mrt_preview.jpeg)
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要