Paracoccidioides brasiliensis induces neutrophil extracellular traps in vitro

Event Abstract Back to Event Paracoccidioides brasiliensis induces neutrophil extracellular traps in vitro Tatiana F. Bachiega1*, Reginaldo K. Fernandes1, Daniela R. Rodrigues1, Helanderson A. Balderramas1, Luciane A. Dias-Melicio2 and Ângela M. Soares1* 1 Universidade Estadual Paulista "Julio de Mesquita Filho" - UNESP, Departamento de Microbiologia e Imunologia, Brazil 2 Universidade Estadual Paulista "Julio de Mesquita Filho" - UNESP, Departamento de Patologia, Brazil Paracoccidioides brasiliensis is a dimorphic fungus that is the causative agent of paracoccidioidomycosis, a chronic, subacute or acute mycosis, with visceral and cutaneous involvement. Recently, studies have focused on the role of neutrophils that are involved in primary response to the fungus. In addition to their ability to eliminate pathogens by phagocytosis and antimicrobial secretions, it has been shown recently that neutrophils can trap and kill microorganisms by release of extracellular structures composed by DNA and antimicrobial proteins, called neutrophil extracellular traps (NETs).The aim of this study was to demonstrate whether P. brasiliensis induces NETs release by human neutrophils activated or not with cytokines that promote fungicidal activity of these cells. Human neutrophils were isolated from peripheral blood of healthy donors and incubated with P. brasiliensis for 60, 90, 120 and 150 minutes. Some of these cultures were pré-treated with DNAse (to degrade NETs) or PMA (positive control of NETs release). Samples analyzed by scanning electron microscopy and photomicrographs demonstrated that P. brasiliensis induces NETs 60, 90, 120 and 150 minutes after interaction with neutrophils. DNAse treatment degraded these structures, whereas PMA induced their formation. Moreover, by using confocal imunofluorescence microscopy the following NET constituents were identified in activated cells with TNF-α, IFN-γ and GM-CSF: DNA (stained with DAPI, elastase (stained with FITC) and histones (stained with TEXAS RED). Thus, we demonstrated that Paracoccidioides brasiliensis is able to induce NETs. Acknowledgements FAPESP 2010/17405-5; FAPESP 2010/18957-1 Keywords: Paracoccidioides brasiliensis, neutrophil extracellular traps, confocal imunofluorescence microscopy, Scanning electron microscopy, innate immunity Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Host-pathogen interactions Citation: Bachiega TF, Fernandes RK, Rodrigues DR, Balderramas HA, Dias-Melicio LA and Soares ÂM (2013). Paracoccidioides brasiliensis induces neutrophil extracellular traps in vitro. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00502 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 29 Apr 2013; Published Online: 22 Aug 2013. * Correspondence: Miss. Tatiana F Bachiega, Universidade Estadual Paulista "Julio de Mesquita Filho" - UNESP, Departamento de Microbiologia e Imunologia, Botucatu, São Paulo, 18618970, Brazil, tatybachiega@hotmail.com Prof. Ângela M Soares, Universidade Estadual Paulista "Julio de Mesquita Filho" - UNESP, Departamento de Microbiologia e Imunologia, Botucatu, São Paulo, 18618970, Brazil, acsoares@ibb.unesp.br Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Tatiana F Bachiega Reginaldo K Fernandes Daniela R Rodrigues Helanderson A Balderramas Luciane A Dias-Melicio Ângela M Soares Google Tatiana F Bachiega Reginaldo K Fernandes Daniela R Rodrigues Helanderson A Balderramas Luciane A Dias-Melicio Ângela M Soares Google Scholar Tatiana F Bachiega Reginaldo K Fernandes Daniela R Rodrigues Helanderson A Balderramas Luciane A Dias-Melicio Ângela M Soares PubMed Tatiana F Bachiega Reginaldo K Fernandes Daniela R Rodrigues Helanderson A Balderramas Luciane A Dias-Melicio Ângela M Soares Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.