High-Content Screening in Cell Biology

Cell-based screening assays are frequently used in drug discovery and academic research to identify cellular phenotypes in response to a genetic or chemical perturbation. When investigating cell biology processes, the use of high-content imaging facilitates the analysis of a large number of samples within a reasonably short time frame and at moderate costs. Here, we will provide an overview of the high-content screening process, from experimental design to available platform technologies and bioinformatics analysis.