Plant regeneration of A. lakoocha from encapsulated nodal explants

One of the alternative methods adopted in recent ye ars is to use biotechnological approaches for impro ving the tree species. A proficient protocol for encapsulation of nodal segments of Artocarpus lakoocha Roxb has bee n developed for plant regeneration through non-embryogenic synt hetic seeds. Concentrations of sodium alginate and calcium chloride greatly affected morphology and texture en capsulating gel. 3 % sodium alginate with 100 mM Ca Cl 2 has been found to be best possible concentration for th e production of identical synthetic seeds. 25 days old in vivo plantlet of A. lakoocha was used for obtaining noda l segments, used in all experiments. Five experime nts were performed using MS medium (agar solidified and liqu id) supplemented with 1, 3 and 5 mg/l BAP. Concentr ation of 1 mg/l BAP was observed to be more effective for ac hieving highest regeneration frequency in compariso n to other concentration of BAP (3 and 5 mg/l). In third exper iment only different strength of MS medium was used , in last two experiments nodal segments prior encapsulation and encapsulated nodal segments were given pulse treatm ent with (IBA)for 48 hours and concentration of 3 mg/l BAP was observed to be most effective for synthetic se ed regeneration . Data were recorded after 4 weeks of culture.