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计时电流法研究4-(N-亚硝基甲氨基)-1-(3-吡啶基)-1-丁酮对谷氨酸脱氢酶活性的影响

You-an MAO,Ke-jun ZHONG,Wan-zhi WEI,Xin-liang WEI,Hong-bing LU

Journal of Analytical Science（2005）

Cited 22|Views1
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Abstract
用计时电流法研究了烟草特有亚硝胺之一4-(N-亚硝基甲氨基)-1-(3-吡啶基)-1-丁酮(NNK)对谷氨酸脱氢酶(GLDH)催化活性的影响,测定了NNK存在与否时GLDH酶促反应的最大反应速率及米氏常数或表观米氏常数.实验结果表明,NNK对GLDH的催化活性有明显抑制作用,而且属于可逆竞争性抑制,测得在25℃,pH 8.0时抑制常数Ki为176 μmol·L-1.
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Key words
Glutamate Decarboxylase
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