Antitumor Activity Of Amitosin

The influence of different concentrations of Amitosin and Isatison on melanoma B-16 was tested in vitro using the method of Comets and in vivo on linear mice C57B16. It was established that the appliance of Amitosin concentration 100 mu g/ml led to the minimal length of the tail (15 px) of damaged DNA molecule and to the minimal percentage of DNA in the tail (59.08 px) as compared with other concentration indicating that melanoma cells' DNA was less damaged. It was demonstrated that Amitosin has maximal positive effect on the survival and even recovery of animals with melanoma it was established that Amitosin application leads to the highest level of tumor growth inhibition (57.21%) that is 15% higher as compared with Izatison. The combined effect of both inhibition by approximately 34%; the level of metastasis is about 7%.