HPLC fingerprint of total flavonoids extracts from Ampelopsis megalophylla.

[Objective]To establish the HPLC fingerprint of total flavonoids extracts from Ampelopsis megalophylla. [Methods] Total flavonoids extracts were obtained from A. megalophylla by reflux extraction with 70% ethanol. Analysis was carried out by RP- HPLC. Chromatographic column was Angilent TC-C18（4.6 mm × 250 mm,5 μm）; mobile phase was acetonitrile-0.2% phosphoric acid solution; column temperature was 30 ℃; detection wavelength was 265 nm; and injection volume was 20 μL. [Results]Fingerprints of 11 batches of total flavonoids extracts from A. megalophylla were detected. 10 common fingerprint peaks were found out. Results of methodological study met the requirements of fingerprint technology. [Conclusions]HPLC fingerprint of total flavonoids extracts from A. megalophylla were established,providing references for the quality control of total flavonoids extracts from A. megalophylla.