Spr Dispersion Compensation For A Gold-Coated Corrugated Surface

The objective of this effort is to improve upon a microarray-based system for label-free, high-throughput proteomic analysis. The system operation is based on a novel grating-coupled surface plasmon resonance imaging (GCSPRI) technology that enables measurement of hundreds to possibly thousands of binding events simultaneously without the limitations of reporter molecules. We expect that this technology will provide a powerful new tool for highly multiplexed analysis of an organism's global protein profile, reflecting metabolic and physiologic activity in relation to time, development and interaction with the environment. It will be applicable to the detection of a broad range of metabolic products, signaling molecules, hormones, enzymes, receptors and other proteins. We present improvements by way of SPR-dispersion compensation with a properly oriented transmission grating placed in the excitation path. This illumination technique is broad-band which reduces the effects of thin-film interference, permitting a 20-fold decrease in sample volume as well as providing more interrogation light.