Advanced digital methods for blood flow flux analysis using µPIV approach

Proceedings of SPIE(2015)

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Abstract
A digital optical system focused on work with laboratory animals for intravital capillaroscopy has been developed. It implements the particle image velocimetry (PIV) based approach for measurements of red blood cells velocity in laboratory rat stomach capillaries. We propose a method of involuntary displacement compensation of the capillary network images. Image stabilization algorithm is based on correlation of feature tracking. The efficiency of designed image stabilization algorithm was experimentally demonstrated. The results of capillary blood flow analysis are demonstrated.
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Key words
intravital mu PIV,blood flow flux,digital image processing,native capillaries network,image stabilization,Euclidean skeleton,optical clearing agent,capillaries blood flow velocity
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