Flash chromatography guided fractionation and antibacterial activity studies of Angelica archangelica root extracts.

Flash chromatography of Methanol, Methanol Water (1:1) and Water extracts of the roots of Angelica archangelica was carried out. The various fractions obtained were subjected to antibacterial activity against four clinically significant strains using Ciprofloxacin (antibiotic) as standard with fractions from methanol extract showing promising activity. One more interesting result was that all the ethyl acetate fractions from Methanol, Methanol Water (1:1) and Water extracts were more active. So these fractions can be further phytochemically investigated for the isolation of biologically active constituents. The positive biological activities manifested by the fractionated extracts from crude extracts render concrete substantiation for the use of such species for traditional medicinal purposes. Keywords- Angelica archangelica, Antibacterial activity, Flash chromatography, Fractionation, MIC