Quality Evaluation Of Atlantic Halibut (Hippoglossus Hippoglossus L) During Ice Storage Using H-1 Nmr Spectroscopy

H-1 nuclear magnetic resonance (NMR) spectroscopy has been investigated as a possible tool for quality evaluation of Atlantic halibut (Hipoglossus hipoglossus L). Perchloric acid extracts of muscle samples, taken from chill-stored fish over a period of three weeks, were analysed with NMR. TMAO was not found to change notably with storage time, whereas phosphocreatine was only detectable in samples taken at the day of slaughter, NADH(+) was detectable in samples up to two days after slaughter. ATP and its degradation products were monitored during the whole experiment, which enabled calculations of K-values, K-values derived from NMR data was compared to K-value measurements with Fresh Tester. The K-values found in the NMR analyses were low during the whole period. These experiments have proven that NMR is a possible method for studying ATP degradation of Atlantic halibut, with the potential of monitoring several metabolites simultaneously.