Tilling In Lotus Japonicus

Following the seminal work on TILLING in Arabidopsis thaliana, a population of EMS-mutagenized plants was established for Lotus japonicus 'Gifu' to be used for both forward and reverse screening. This was developed into the Lotus TILLING platform which subsequently became RevGenUK covering not only TILLING in L. japonicus, but also Medicago truncatula and other species. Over the last 10 years, nearly two thousand mutations for more than 160 genes have been identified using the platform. In this article, we cover the history, development and current technology employed in the process and explore the impact TILLING has had on Lotus research.