Association Studies of 3 Candidate Genes with Type 2 Diabetes Mellitus in a Chinese Population

Objectives To explore the relationship between the polymorphisms of the select-ed short tandem repeats (STRs) of the candidate genes and type 2 diabetes mellitus (DM) in a Chinesepopulation, the role of genetic and environmental factors in the development of type 2 diabetes. Meth-ods STRs including D11S916 of uncoupling protein 3 (UCP3) gene,binucleotide repeat (CA). with-in intron 6 [HSLi6 (CA)n] of hormone- sensitive lipase(HSL) gene and D20S501 of protein tyrosinephosphatase- 1B (PTP-1B) gene polymorphisms were detected by polymerase chain reaction (PCR) , poly-acrylamiie gel electrophoresis and silver staining in 106 patients with type 2 DM and 102 control sub-jects. Results The allele distribution of UCP3 and HSL gene differed significantly between patientswith type 2 diabetes and control subjects (χ2 = 26. 12, P＜0.005; χ2=10. 33, P＜0. 005, respec-tively). For UCP3 and HSL gene,the frequencies of alleles A6,A7 ,A8 and allele B9 were much high-er in diabetic patients than in control subjects (0. 090 vs 0. 020,P＜0. 005; 0. 109 vs 0. 015,P＜0. 005; 0. 033 υs 0. 000,P＜0.05; 0. 033 υs 0. 005,P＜0. 05,respectively),while the fre-quencies of allele A1 and allele B5 were lower in diabetic patients than in control subjects (0. 090 vs0. 206,P＜0. 005; 0. 057 vs 0. 118,P＜0. 05,respectively). At D20S501 locus,The allele dis-tribution of PTP-1B gene had no significant difference in two groups (χ2=3. 77 ,P＞0. 05). Multi-variate logistic regression analysis showed positive correlation between alleles A 6,A 7 of UCP3 gene,sys-tolic blood pressure , apolipoprotein B , lipoprotein (a) and type 2 diabetes. Conclusion Our datashow that D11S916 of UCP3 gene and HSLi6 (CA), of HSL gene polymorphisms are associated withtype 2 diabetes in Chinese suggesting that UCP3 and HSL might represent susceptibility genes for type 2diabetes. D20S501 of PTP-1B gene polymorphism isnot associated uith type 2 diabetes in Chinese. AllelesA6, A7 of UCP3 gene, systolic blood pressure,apolipoprotein B, lipoprotein (a) may play same rolein the development of type 2 diabetes.