Technical variability of 2D gel electrophoresis – Application to soybean allergens

•2-DE is robust and suitable for comparing the GM soybean with its non-GM counterpart; its technical variability is lower than the biological variability.•Main source of variability is the gels so 3–4 gel replicates should be used.•Other sources of variability are minor, which gives some experimental flexibility, i.e. study can be run over several days, several operators.