Alpha-Glucosidase Inhibitory Activity And Antioxidant Capacity In The Peel And Pulp Of Mixed-Species Blueberry Hybrids

Inhibition of alpha-glucosidase activity is considered an effective means for controlling diabetes by regulating glucose uptake, and blueberries have been shown to possess high levels of inhibitory activity. In the present study, we examined the variations in alpha-glucosidase inhibition, phenolic and anthocyanin levels, and antioxidant capacity in the peel and pulp of 16 mixed-species rabbiteye hybrids (Vaccinium ashei ReadexVaccinium spp.), one rabbiteye cultivar (V. ashei) and two highbush hybrids (Vaccinium corymbosum). Peel tissue had, on average, about four times higher levels of alpha-glucosidase inhibitory activity than pulp, and exhibited significantly higher levels of all other measured activities, even though the peel comprised only a small portion of the fruit. Significant variations in the levels of antioxidant activity were observed; however, no consistent differences were observed between the hybrids with various species composition. Significant positive correlations (r >= 0.84) were found among -glucosidase inhibitory activity, total anthocyanin (TA) and phenolic levels, and scavenging activity against ROO., center dot OH, O-1(2) and H2O2 radicals in the extracts from the peel and pulp. There was a high correlation observed between alpha-glucosidase inhibitory activity levels and ROO.(ORAC)(peel) (r=0.95). A similarly high correlation with TA(peel) (r=0.93) suggests that TA would be a suitable assay proxy if a broader genotypic evaluation of blueberry genotypes is desired.