Successful delivery derived from the cryopreservation of individually selected sperm from patients with Klinefelter's syndrome with assisted oocyte activation (AOA): two years follow up case report

ObjectiveTo report a new technique for freezing individually isolated spermatozoa from patients with Klinefelter's syndrome with assisted oocyte activation (AOA).DesignRetrospective analize.Materials and MethodsWe found a total of 26 immotile spermatozoa following Micro Dissection-TESE. Nine spermatozoa were individually picked up using a micro injector and frozen on a Cryotip (KITAZATO Biopharma) device and remaining spermatozoa were frozen using conventional method aliquoted into 3 cryovials. The spermatozoa were slow cooled in booth techniques using Sperm Freeze™ solution (FertiPro N.V.) Assisted oocyte activation (AOA) was performed either by exposure to 5 uM ionomycin for 1min or electroporation with a single direct current 30-35 V for 99μs.ResultsWe obtained 8 mature oocytes. The recovery rate from the Cryotips was 88.9% (8/9). However, only one sperm was recovered from the Cryovial group and this sperm was not suitable for ICSI because this sperm was immotile. We performed ICSI with AOA. Normal fertilization rate was 75% (6/8) and on the day3, we transferred a 8 cell stage embryo and achieved a singleton pregnancy which subsequently resulted in full-term delivery of a healthy boy 2700g. The boy is now 2 years and 6 months old, weighing 11 kg and with a height of 86 cm. The boy has grown up without any developmental disabilities.ConclusionSuccessful delivery derived from the cryopreservation of individually selected sperm from patients with Klinefelter's syndrome with AOA. Genetic counseling should be given prior to ICSI-AOA and long term follow-up of children born from these treatments are also necessary. ObjectiveTo report a new technique for freezing individually isolated spermatozoa from patients with Klinefelter's syndrome with assisted oocyte activation (AOA). To report a new technique for freezing individually isolated spermatozoa from patients with Klinefelter's syndrome with assisted oocyte activation (AOA). DesignRetrospective analize. Retrospective analize. Materials and MethodsWe found a total of 26 immotile spermatozoa following Micro Dissection-TESE. Nine spermatozoa were individually picked up using a micro injector and frozen on a Cryotip (KITAZATO Biopharma) device and remaining spermatozoa were frozen using conventional method aliquoted into 3 cryovials. The spermatozoa were slow cooled in booth techniques using Sperm Freeze™ solution (FertiPro N.V.) Assisted oocyte activation (AOA) was performed either by exposure to 5 uM ionomycin for 1min or electroporation with a single direct current 30-35 V for 99μs. We found a total of 26 immotile spermatozoa following Micro Dissection-TESE. Nine spermatozoa were individually picked up using a micro injector and frozen on a Cryotip (KITAZATO Biopharma) device and remaining spermatozoa were frozen using conventional method aliquoted into 3 cryovials. The spermatozoa were slow cooled in booth techniques using Sperm Freeze™ solution (FertiPro N.V.) Assisted oocyte activation (AOA) was performed either by exposure to 5 uM ionomycin for 1min or electroporation with a single direct current 30-35 V for 99μs. ResultsWe obtained 8 mature oocytes. The recovery rate from the Cryotips was 88.9% (8/9). However, only one sperm was recovered from the Cryovial group and this sperm was not suitable for ICSI because this sperm was immotile. We performed ICSI with AOA. Normal fertilization rate was 75% (6/8) and on the day3, we transferred a 8 cell stage embryo and achieved a singleton pregnancy which subsequently resulted in full-term delivery of a healthy boy 2700g. The boy is now 2 years and 6 months old, weighing 11 kg and with a height of 86 cm. The boy has grown up without any developmental disabilities. We obtained 8 mature oocytes. The recovery rate from the Cryotips was 88.9% (8/9). However, only one sperm was recovered from the Cryovial group and this sperm was not suitable for ICSI because this sperm was immotile. We performed ICSI with AOA. Normal fertilization rate was 75% (6/8) and on the day3, we transferred a 8 cell stage embryo and achieved a singleton pregnancy which subsequently resulted in full-term delivery of a healthy boy 2700g. The boy is now 2 years and 6 months old, weighing 11 kg and with a height of 86 cm. The boy has grown up without any developmental disabilities. ConclusionSuccessful delivery derived from the cryopreservation of individually selected sperm from patients with Klinefelter's syndrome with AOA. Genetic counseling should be given prior to ICSI-AOA and long term follow-up of children born from these treatments are also necessary. Successful delivery derived from the cryopreservation of individually selected sperm from patients with Klinefelter's syndrome with AOA. Genetic counseling should be given prior to ICSI-AOA and long term follow-up of children born from these treatments are also necessary.