Shaken flasks by resonant acoustic mixing versus orbital mixing: Mass transfer coefficient kLa characterization and Escherichia coli cultures comparison
Biochemical Engineering Journal(2016)
摘要
•Resonant acoustic mixing (RAM) for shaken flasks is characterized in terms of the kLa.•Empirical models for kLa in shaken flasks by RAM and orbital mixing are presented.•E. coli cultures show similar kinetics in RAM and orbital mixing at kLa of 46h−1.•At kLa of 92h−1 E. coli exhibited differences in DOT profiles and culture parameters.
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关键词
A,a, b,c,adj R2,A/V,CL*,CL,d,d0,DOT,DOF,Fr,a,kLa,n,Ph,RAM,RMSE,VN,VF,V
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