Nitrosylative Stress During Ischemia–Reperfusion Injury: Implications For Poly (Adenosine Diphosphate-Ribose) Polymerase and Nitric Oxide Synthase Activity

Poly (adenosine diphosphate [ADP]-ribose) polymerase (PARP) inhibition is cytoprotective during limb ischemia–reperfusion (IR) injury. Paradoxically, PARP activation may be triggered by dysfunctional nitric oxide synthase (NOS) activity. These experiments were undertaken to assess whether NOS inhibition will ameliorate skeletal muscle injury and alter PARP activity during the acute and early chronic phases of reperfusion in a mouse model of hind limb IR.