Structural Characterization of Oat Bran (1→3), (1→4)-β-D-Glucans by Lichenase Hydrolysis Through High-Performance Anion Exchange Chromatography with Pulsed Amperometric Detection

Cereal mixed linkages (1 → 3) (1 → 4)-β-D-glucan is a linear polysaccharide composed of glucose units. Oat β-glucan is a natural polymer. The main products of β-glucanase are oligosaccharides with DP3 and DP4, i.e., 3-Ob-cellobiosyl-D-glucose and 3-Ob-cellotriosyl-D-glucose, which represent over 90% of the molecule. Keeping in mind all the benefits of oat bran, the present study was planned to investigate the structural properties of oat bran, high-performance anion exchange chromatography with pulsed amperometric detection was used to examine these oligosaccharides. The structural analysis of oat bran of two oat varieties revealed that the ratio of soluble and insoluble triose to tetraose in β-glucan fraction was 1.44 and 1.78, respectively, for Avon variety; while the ratio of soluble and insoluble triose to tetraose in β-glucan fraction for Sargodha-81 was 1.49 and 1.77. The major units determined were cellotriose and cellotetraose. Other units cellopentaose and hexaoses were also existed but in minor ...